Dual Secured Nano-Melittin for Safe and Effective Eradicating Cancer Cells
نویسندگان
چکیده
Materials: Glycol chitosan (GCS, Mw = 200 kDa; degree of deacetylation = 90%), potassium persulfate, sodium borohydride, succinic anhydride, melittin from bee venom, sodium dodecyl sulfate, D-(+)-trehalose dehydrate, N-succinimidyl 3-[2-pyridyldithio]-propionate (SPDP), tris(2carboxyethyl)phosphine (TCEP), triethlyl amine (TEA), ethylenediaminetetraacetic acid (EDTA), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St Louis, MO, USA). L-cysteine HCl and Ellman’s reagent were purchased from Thermo Scientific (Rockford, IL, USA). Dulbecco’s Modified Eagle Medium (DMEM) supplied with high glucose, trypsin and penicillin-streptomycin were purchased from Invitrogen (Carlsbad, CA, USA). Fetal bovine serum (FBS) was purchased from ATCC (Manassas, VA, USA).
منابع مشابه
بررسی اثر ملیتین بر میزان بیان پروتئین Rac1 به عنوان مارکر متاستاز در سلول‌های سرطان معده رده AGS
Introduction: Understanding the molecular mechanisms of metastasis is important for the design and effective use of novel therapeutic strategies in order to combat metastases. One class of molecules that has been implicated in metastasis is Rac1. Regarding complications of conventional therapies such as chemotherapy, current studies are evaluating natural compounds with anticancer Properties. M...
متن کاملجداسازی ملیتین از زهر زنبور عسل و بررسی تاثیر آن بر رشد سلول‌های سرطان معده
Background: Gastric cancer (GC) is one of the most common cancers worldwide and in Iran. Conventional therapies are surgery and chemotherapy. Current studies are evaluating natural compounds in inhibiting growth of cancer cell. In this study isolated peptide melittin with 26 amino acids from bee venom and its impact on the viability and proliferation of gastric cancer cells was investigated. Me...
متن کاملجداسازی ملیتین از زهر زنبور عسل بومی ایران و بررسی اثر آن بر تکثیر سلول‌های سرطانی دهانه رحم - رده HeLa
Introduction: Cervical cancer is the second prevalent cancer in developing countries and the sixth prevalent cancer in USA. Since conventional treatment methods are associated with detrimental side effects, searching for new drugs using natural ingredients is very important. Previous studies have shown that melittin (main component of honey bee venom) has anticancer properties along with the ef...
متن کاملA novel melittin nano-liposome exerted excellent anti-hepatocellular carcinoma efficacy with better biological safety
Melittin is the main effective component of bee venom and has extensive biological functions; however, serious side effects have restricted its clinical application. Preclinical and clinical studies showed that the main adverse events were allergic reaction and pain at the administration site. To decrease the toxicity, we prepared melittin nano-liposomes by encapsulating melittin with poloxamer...
متن کاملFeMn2O4 nanoparticles coated dual responsive temperature and pH-responsive polymer as a magnetic nano-carrier for controlled delivery of letrozole anti-cancer
Objective(s): For cancer cells, an efficient and selective drug delivery vehicle can remarkably improve therapeutic approaches. This paper focuses on the synthesis and characterization of magnetic MnFe2O4 NPs and their incorporation in a dual temperature and pH-responsive polymer, which can serve as an efficient drug carrier. Materials and Methods: MnFe2O4 NPs were synthesized by chemical co-pr...
متن کامل